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Updated: Aug 20, 2026
Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
Published on: June 29, 2021
Escherichia coli stress protein YciF: expression, crystallization and preliminary crystallographic analysis
Deqian Liu1, Yonghong Zhao, Xiuzhen Fan
1The Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch at Galveston, TX 77555-0647, USA.
Abstract:
The Escherichia coli stress protein YciF was overexpressed and purified by three chromatographic steps. Crystals were obtained using ammonium sulfate as a precipitant. The YciF protein crystals diffracted beyond 2.25 A resolution using a rotating-anode X-ray source. The lattice type is rhombohedral, with unit-cell parameters a = b = 80.0, c = 131.03 A, alpha = beta = 90.0, gamma = 120.0 degrees. The crystal belongs to space group R32.
