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Multi-stage nature of the thermal denaturation process in collagen
V L Shnyrov1, V Ch Lubsandorzhieva, G G Zhadan
1Institute of Theoretical and Experimental Biophysics, U.S.S.R. Academy of Sciences, Pushchino, Moscow region.
Biochemistry International
|February 1, 1992
Abstract:
Thermal denaturation of acid-soluble collagen from polar cod (Eleginus gracialis) skin has been studied by scanning microcalorimetry and intrinsic spectrofluorimetry methods. The thermal denaturation process occurs in three independent stages reflecting the melting of 33 kDa, 97 kDa, and 230 kDa domains. Thermodynamical parameters of the collagen denaturation have been determined.