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Updated: Oct 12, 2026

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
Published on: July 8, 2011
Adenoviral transduction of dendritic cells
Rienk Offringa1, Kitty Kwappenberg, Martijn Rabelink
1Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
Abstract:
Recombinant adenoviral (rAd) vectors are highly suitable for efficient genetic modification of dendritic cells (DC). In certain cases, the high immunogenicity of rAd may be a disadvantage. This chapter describes the essential aspects of optimal rAd-mediated gene transfer into DC, discusses the consequences of the immunogenicity of rAd vectors, and provides suggestions to minimize these complications.
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