Related Experiment Video
Updated: Aug 15, 2026
08:08
Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
Published on: July 8, 2011
Adenoviral transduction of dendritic cells
Rienk Offringa1, Kitty Kwappenberg, Martijn Rabelink
1Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
Methods in Molecular Medicine
|December 9, 2004
Abstract:
Recombinant adenoviral (rAd) vectors are highly suitable for efficient genetic modification of dendritic cells (DC). In certain cases, the high immunogenicity of rAd may be a disadvantage. This chapter describes the essential aspects of optimal rAd-mediated gene transfer into DC, discusses the consequences of the immunogenicity of rAd vectors, and provides suggestions to minimize these complications.
