Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Target selectivity in EF-hand calcium binding proteins.

Shibani Bhattacharya1, Christopher G Bunick, Walter J Chazin

  • 1Department of Biochemistry, Center for Structural Biology, 5140 BIOSCI/MRBIII, Vanderbilt University, Nashville, TN 37232-8725, USA.

Biochimica Et Biophysica Acta
|December 14, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Matching-Adjusted Indirect Comparison of Risankizumab Versus Icotrokinra in Adult Patients with Moderate-to-Severe Plaque Psoriasis.

Dermatology and therapy·2026
Same author

Abrocitinib Achieves Early and Complete/Near-Complete Skin Clearance Plus Itch-Free State in Atopic Dermatitis: Phase 3 Pooled Post Hoc Analysis.

Dermatology and therapy·2026
Same author

<i>Staphylococcus aureus</i> ZigA is implicated in survival in zinc-deplete and genotoxic environments.

Microbiology spectrum·2026
Same author

Understanding the atopic dermatitis-psoriasis phenotypic switch through a pharmacovigilance-based approach.

The Journal of investigative dermatology·2026
Same author

Efficacy of Upadacitinib in Patients with Atopic Dermatitis of the Head and Neck Region.

Dermatology and therapy·2026
Same author

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Nature communications·2026
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

EF-hand calcium binding proteins exhibit diverse functions despite structural similarities. Structural insights reveal how molecular architecture and calcium ion binding fine-tune protein interactions for specific cellular targets.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Background:

  • EF-hand calcium binding proteins share sequence and structural homology.
  • Despite similarities, these proteins display diverse responses to calcium binding and varied biological functions.
  • Atomic resolution structures determined over the last decade have advanced understanding of EF-hand protein sequence optimization.

Purpose of the Study:

  • To review current understanding of structural mechanisms underlying EF-hand protein target selectivity.
  • To demonstrate key concepts using S100 and calmodulin family proteins.
  • To elucidate how functional selectivity arises from a generic ionic signal.

Main Methods:

  • Review of structural data and biochemical properties of EF-hand proteins.

Related Experiment Videos

  • Comparative analysis of calmodulin and S100 protein families.
  • Focus on atomic resolution structures and Ca(2+) ion binding characteristics.

    • Main Results:

    • Three primary factors influence target selectivity: molecular architecture, calcium ion binding response, and target binding surface characteristics.
    • Calmodulin and S100 proteins exemplify how these factors facilitate diverse binding configurations.
    • Structural insights explain how conserved protein families achieve specific cellular targeting.

    Conclusions:

    • Molecular architecture, calcium binding dynamics, and surface complementarity are critical for EF-hand protein target specificity.
    • Comparative studies of calmodulin and S100 proteins highlight mechanisms for achieving functional diversity from conserved structural motifs.
    • Understanding these factors is key to deciphering how EF-hand proteins translate a simple calcium signal into specific cellular actions.