Related Experiment Video
Updated: Aug 20, 2026

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Published on: September 17, 2016
Morphogenetic pathway of spore wall assembly in Saccharomyces cerevisiae
Alison Coluccio1, Edith Bogengruber, Michael N Conrad
1Life Sciences, SUNY Stony Brook, Stony Brook, NY 11794-5215, USA.
Abstract:
The Saccharomyces cerevisiae spore is protected from environmental damage by a multilaminar extracellular matrix, the spore wall, which is assembled de novo during spore formation. A set of mutants defective in spore wall assembly were identified in a screen for mutations causing sensitivity of spores to ether vapor. The spore wall defects in 10 of these mutants have been characterized in a variety of cytological and biochemical assays. Many of the individual mutants are defective in the assembly of specific layers within the spore wall, leading to arrests at discrete stages of assembly. The localization of several of these gene products has been determined and distinguishes between proteins that likely are involved directly in spore wall assembly and probable regulatory proteins. The results demonstrate that spore wall construction involves a series of dependent steps and provide the outline of a morphogenetic pathway for assembly of a complex extracellular structure.
Related Concept Videos
Gene Regulation During Sporulation
Role of Microtubules in Cell Wall Deposition
Yeast Signaling
Archaeal Cell Wall
Endospores and Sporulation
The Phragmoplast
The...

