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Updated: Aug 20, 2026
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Published on: July 31, 2012
Construction of a eukaryotic expression plasmid of Humanin
Objective:
To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.
Methods:
The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.
Results:
Recombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence.
Conclusions:
A eukaryotic expression plasmid of Humanin was successfully constructed.
