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Encephalomyocarditis (EMC) virus-induced sialodacryoadenitis in mice.
Atsuko Ohguchi1, Yumi Nakayama, Chiyo Doi
1Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
Experimental and Molecular Pathology
|December 15, 2004
Summary
Encephalomyocarditis virus D variant infects salivary and lacrimal glands, targeting acinar cells. This viral tropism explains the observed sialodacryoadenitis in mice.
Area of Science:
- Virology
- Pathology
- Immunology
Background:
- Encephalomyocarditis virus (EMC-D) can cause acute sialodacryoadenitis.
- Mouse strain sensitivity to EMC-D virus varies, impacting disease outcomes.
Purpose of the Study:
- To investigate the occurrence and tissue tropism of EMC-D virus in salivary and lacrimal glands.
- To correlate viral load and histopathological changes with mouse strain sensitivity.
Main Methods:
- Intranasal inoculation of three mouse strains (C57BL/6, BALB/c, DBA/2) with EMC-D virus.
- Analysis of viral RNA, blood virus titers, and histopathological changes in salivary and lacrimal glands.
Main Results:
- EMC-D virus RNA and lesions were detected in parotid, intraorbital, and extraorbital lacrimal glands, specifically in acinar cells.
- Initial lesions showed acinar cell pyknosis, progressing to coagulative necrosis and inflammation in susceptible mice and higher doses.
- Ductal epithelial cells were unaffected, indicating specific cell tropism.
Conclusions:
- EMC-D virus exhibits distinct tissue and cell tropism within salivary and lacrimal glands.
- Acinar cell susceptibility is likely mediated by viral receptor distribution.
- Understanding this tropism is crucial for studying EMC-D virus pathogenesis.