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MultiPLX: automatic grouping and evaluation of PCR primers
Lauris Kaplinski1, Reidar Andreson, Tarmo Puurand
1Department of Bioinformatics, University of Tartu, Tartu, Estonia.
Unlabelled:
MultiPLX is a new program for automatic grouping of PCR primers. It can use many different parameters to estimate the compatibility of primers, such as primer-primer interactions, primer-product interactions, difference in melting temperatures, difference in product length and the risk of generating alternative products from the template. A unique feature of the MultiPLX is the ability to perform automatic grouping of large number (thousands) of primer pairs.
Availability:
Binaries for Windows, Linux and Solaris are available from http://bioinfo.ebc.ee/download/. A graphical version with limited capabilities can be used through a web interface at http://bioinfo.ebc.ee/multiplx/. The source code of the program is available on request for academic users.
Contact:
maido.remm@ut.ee.
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