Related Experiment Videos
[Excision of selectable marker gene from transgenic plant]
1Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. gongzx@sumn.shcnc.ac.cn
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao = Journal of Plant Physiology and Molecular Biology
|December 16, 2004
Summary
Generating marker-free transgenic plants is crucial for public acceptance. This research reviews techniques like segregation and recombination excision for removing selectable marker genes from genetically modified crops.
Area of Science:
- Plant biotechnology
- Molecular biology
- Genetics
Context:
- Traditional plant transformation relies on selectable marker genes (e.g., antibiotic/herbicide resistance) for identifying transformed cells.
- The presence of these markers in food crops raises public concern regarding safety and environmental impact.
Purpose:
- To review and discuss methods for generating selectable marker-free transgenic plants.
- To highlight advances in plant biotechnology for creating genetically modified organisms without antibiotic or herbicide resistance genes.
Summary:
- Discusses segregation and recombination excision techniques for removing selectable marker genes after plant transformation.
- Reviews current advancements in producing marker-free transgenic plants, addressing public concerns.
Impact:
- Facilitates the development of safer and more publicly accepted genetically modified crops.
- Advances plant biotechnology by enabling the production of transgenic plants without the need for persistent selectable markers.