[The application of computer assisted sperm analysis on in vitro fertilization]

To set up the prognostic parameters for clinical use of IVF-ET technique from the male side, the influence of the quantitative parameters about sperm motility on human in vitro fertilization rate was evaluated in this study.

The sperm samples were classfied into three groups in accordance to the eggs' in vitro fertilization rate of > or =50%, <50% and zero. Besides,based on the achievement of pregnancy after embryo transfer, the samples were divided into two groups: pregnancy achieved and pregnancy not achieved. Then we adopted the system of computer assisted sperm analysis (CASA) in assessing some parameters about sperm movement. The correlation between them and IVF rate or pregnancy was analyzed with t-test.

This study embraced 69 IVF cycles, among which 64 cycles were completed with 20 cases of pregnancy, including biochemical and clinical pregnancies. The data on sperm movement parameters showed significant difference in Progressive motility, VAP, VSL, VCL and Percent normal sperm morphology between the > or =50% group and the <50% and zero groups (P < 0.01). Significant difference in the above parameters was also observed between the pregnancy positive and negative groups (P < 0.01). Besides, there were significant differences in Sperm movement parameters and Percent normal morphology between pre- and post-washed groups (P < 0.05).

This study has demonstrated that the success rate of "test tube baby" is tightly related to some sperm movement parameters, thus suggesting VAP, VSL and VCL can serve as prognostic parameters for clinical application of IVF.