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Published on: July 9, 2014
[The use of FQ-PCR in detecting condyloma acuminata]
Hua Xie1, Guifang Qin, Song Cheng
1Guangxi Province Public Hospital, Nanning 530021, China.
Objective:
To assess the value of flurogenic quantitative polymerase chain reaction (FQ-PCR) in detecting condyloma acuminatum(CA).
Methods:
Thirty-six samples of CA pathologically diagnosed as positive cases and 84 samples of healthy controls were tested with FQ-PCR, and another 273 samples of clinical cases were examined using both pathological method and FQ-PCR. Then FQ-PCR as a diagnostic test was evaluated.
Results:
FQ-PCR detected HPV6, HPV11 DNA in all the 36 CA samples, the mean copy being 1.0 x 10(7) +/- 10(2)/ml; the 84 samples of the healthy group ware all negative, the mean copy being 3 x 10(1) +/- 2 x 10(1)/ml. The coincidence rate of FQ-PCR with the pathological diagnosis was 100%. There was significant differnce in copy amount between the CA group and the healthy group, (P < 0.05). At the second stage, comparison between FQ-PCR and the pathological diagnosis showed: Se was 100%, Sp 92%, alpha was 0.08, beta 0, accuracy was 98.9%, positive predictive value was 98.8%, negative predictive value 100%; positive likelihood ratio was 12.5, and negative likelihood ratio 0.
Conclusion:
FQ-PCR as a rapid and simple method could accurately detect and quantify CA and its Se and Sp were high; it can be taken as an index in early CA diagnosis.
