Hepatocyte culture in bioartificial livers with different membrane characteristics
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Updated: Aug 20, 2026
"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
Published on: February 19, 2019
Qin Meng1, Guoliang Zhang, Danqing Wu
1Department of Chemical Engineering and Biochemical Engineering, Zhejiang University, 38 Zheda Road, Hangzhou, Zhejiang 310027, PR China. mengq@zju.edu.cn
Abstract:
Rat hepatocytes were cultured in three polysulfone, hollow-fiber cartridges, characterized by two membrane variables: pore size and inner diameter (ID). Hepatocytes entrapped in a micro-filtration (MF) cartridge with the membrane pore size 0.1 microm had twice the production of urea and 4-fold the amount of albumin in comparison to the control cartridge, a ultra-filtration (UF) cartridge with a molecular weight cut-off (MWCO) of 100 kDa. Hepatocytes entrapped in a UF cartridge with ID of 0.5 mm secreted twice the amount of urea and 10-fold the amount of albumin compared with the control UF cartridge.
