Related Experiment Video
Updated: Aug 20, 2026
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Development of an HPLC method for monitoring of Photofrin II therapy
Michael Vogeser1, Moshe Schaffer, Emil Egeler
1Institute of Clinical Chemistry, Hospital of the University of Munich, Munich, Germany.
Objectives:
To develop and to validate a method to quantify plasma porphyrins after application of Photofrin II, a drug preparation that is used for photodynamic therapy (PDT) and as a radiosensitizer.
Design And Methods:
After solid phase extraction of postdose plasma samples, reversed phase high performance-liquid chromatography with fluorescence detection was performed. Plasma porphyrin concentrations were monitored in five patients that were treated with Photofrin II.
Results:
The method proved linear over a wide concentration range, precise, and applicable in a routine clinical laboratory setting.
Conclusions:
The method described here will enable large-scale clinical pharmacokinetic studies on Photofrin II; investigations can now address possible correlations between individual concentrations of drug-derived plasma porphyrins and systemic photosensitivity--representing the main side effect of Photofrin II--with the perspective of individualized light protection regimens after Photofrin II administration.
