4-Hydroxynonenal modulation of p53 family gene expression in the SK-N-BE neuroblastoma cell line

Related Experiment Video

Updated: Aug 20, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Stefano Laurora1, Elena Tamagno, Federica Briatore

  • 1Department of Experimental Medicine and Oncology, Section of General Pathology, University of Turin, Corso Raffaello 30, 10125 Turin, Italy.

Free Radical Biology & Medicine
|December 21, 2004
PubMed

Insights

4-Hydroxynonenal (HNE), a lipid peroxidation product, inhibits neuroblastoma cell proliferation by increasing p53 family proteins and apoptosis-related gene expression. This study reveals HNE

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • 4-Hydroxynonenal (HNE) is a cytotoxic product of lipid peroxidation.
  • The p53 tumor suppressor protein family, including p53, p63, and p73, regulates cell cycle and apoptosis.
  • SK-N-BE human neuroblastoma cells express all three p53 family members.

Purpose of the Study:

  • To investigate the effects of HNE on proliferation, differentiation, and apoptosis in SK-N-BE cells.
  • To determine the impact of HNE on the expression of p53 family proteins (p53, p63, p73) and their target genes (p21, bax).
  • To elucidate the role of HNE in modulating cell cycle regulators like G1 cyclins and pRb phosphorylation.

Main Methods:

  • Treatment of SK-N-BE neuroblastoma cells with HNE and retinoic acid (RA) as a control.
  • Analysis of cell proliferation, cell cycle phase distribution (S-phase), differentiation markers, and apoptosis.
  • Quantitative assessment of p53, p63, p73, p21, and bax protein and gene expression.
  • Evaluation of cyclin D2 expression and pRb protein phosphorylation.

Main Results:

  • HNE inhibited cell proliferation and decreased the proportion of S-phase cells without inducing differentiation.
  • HNE significantly increased the number of apoptotic cells.
  • HNE upregulated the expression of p53, p73, p63, p21, and bax at various time points.
  • HNE downregulated cyclin D2 expression and reduced pRb phosphorylation.

Conclusions:

  • HNE effectively inhibits neuroblastoma cell proliferation.
  • The antiproliferative effect of HNE is mediated through the induction of p53 family proteins and their downstream targets involved in cell cycle arrest and apoptosis.
  • HNE represents a potential therapeutic agent targeting cell cycle progression and inducing apoptosis in neuroblastoma.

More Related Videos

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells09:37

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Published on: August 25, 2021

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas07:00

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas

Published on: February 28, 2019

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...