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Related Experiment Videos

Vacuum packing: a model system for laboratory-scale silage fermentations.

H E Johnson1, R J Merry, D R Davies

  • 1Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth, Ceredigion, UK.

Journal of Applied Microbiology
|December 22, 2004
PubMed
Summary

Vacuum-packed polythene bags offer a cost-effective alternative for lab-scale silage studies, mimicking fermentation in glass vessels. This method allows for flexible packing densities, providing a realistic model system for silage evaluation.

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Area of Science:

  • Agricultural Science
  • Food Science
  • Microbiology

Background:

  • Traditional lab-scale silage studies often utilize fixed-volume glass vessels.
  • These methods can be inflexible and costly for high-throughput sample analysis.

Purpose of the Study:

  • To evaluate vacuum-packed polythene bags as a cost-effective and flexible alternative to glass vessels for lab-scale silage studies.
  • To assess the impact of variable packing densities on silage fermentation within vacuum-packed systems.

Main Methods:

  • Perennial ryegrass and red clover were ensiled in both glass tubes and vacuum-packed polythene bags for 35 days.
  • Fermentation was assessed via pH measurement.
  • The effect of four different initial packing densities (0.397–0.534 g cm⁻³) on silage fermentation was studied over 16 days in vacuum-packed bags.

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Main Results:

  • Similar fermentation patterns, indicated by pH, were observed in both glass and vacuum-packed silos over 35 days.
  • Varying vacuum settings (packing densities) significantly influenced pH decline and lactate accumulation.
  • Gas accumulation was evident in all bags, with volume changes correlating to initial packing density.

Conclusions:

  • Vacuum-packed silos serve as a realistic and effective model system for lab-scale silage fermentation studies.
  • This method offers advantages including higher sample throughput, consistent packing, and the ability to investigate packing density effects and material variations.