A histochemical study of denervated marsupial hemidiaphragm

The marsupial (quokka) hemidiaphragm showed postdenervation hypertrophy and subsequent atrophy. The type II muscle fibres hypertrophied up to 20 days postoperation and then regressed. However, the type I fibres hypertrophied throughout the experimental period (100 days) studied. Unlike denervated eutherian hemidiaphragm, fibre-splitting was absent in the denervated marsupial muscle. An enhancement of the ATPase reaction in the denervated type I fibres may be due to dedifferentiation. Presumably innervation exerts a 'negative control' and prevents increase of type I fibre size in the normal hemidiaphragm.