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Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
Published on: May 29, 2020
[Initial discussion of mice acute hepatic injury caused by Tripterygium glycosides]
Bo Peng1, Ming-san Miao, Yu-liang Wang
1Hepatopathy Institute of Henan Traditional Chinese Medicine, Zhengzhou 450008, Henan, China. pbsir@sina.com
Objective:
To discuss the mechanism of mice acute hepatic injury caused by tripterygium glycosides tablets with different dose at different time.
Method:
Mice were given Tripterygium Glycosides respectively with the dosage of 10 times, 20 times, 30 times of clinical dose to observe the change of mice acute hepatic injury with different does; then, the acute hepatic injury mice were duplicated with 20 times clinical dosage and mice serum ALT were detected at 9, 18, 27, 36 h to observe the change of mice acute hepatic injury at different time. The activity of SOD, GSH-Px in serum and the level of LPO in liver homogenate wevedetected to discuss the mechanism of mice acute hepatic injury caused by Tripterygium Glycosides tablets; and liver tissue pathology was observed.
Result:
The acute hepatic injury was obvious with 20 times adult dosage in 18 hours and the acute hepatic injury mice death rate was low.
Conclusion:
Tripterygium Glycosides tablets can cause acute hepatic injury to mice and its mechanism is related to Lipid peroxidation reaction.
Insights
Tripterygium glycosides tablets can cause acute hepatic injury in mice, primarily through lipid peroxidation. This liver damage is dose- and time-dependent, with significant effects observed at 20 times the clinical dose within 18 hours.
Area of Science:
- Pharmacology
- Toxicology
- Hepatology
Background:
- Tripterygium glycosides are used clinically but can cause adverse effects.
- Understanding the mechanism of drug-induced liver injury is crucial for patient safety.
Purpose of the Study:
- To investigate the mechanism of acute hepatic injury induced by Tripterygium glycosides tablets in mice.
- To evaluate the dose- and time-dependent effects of Tripterygium glycosides on liver injury.
Main Methods:
- Mice were administered varying doses (10x, 20x, 30x clinical dose) of Tripterygium glycosides.
- Liver injury was assessed at different time points (9, 18, 27, 36 h) post-administration.
- Serum alanine aminotransferase (ALT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, and liver homogenate lipid peroxidation (LPO) levels were measured.
- Liver tissue pathology was examined.
Main Results:
- Significant acute hepatic injury was observed in mice at 20 times the clinical dose within 18 hours.
- The mortality rate among injured mice remained low.
- Elevated LPO levels indicated oxidative stress contributing to liver damage.
Conclusions:
- Tripterygium glycosides tablets can induce acute hepatic injury in mice.
- The mechanism underlying this injury is linked to lipid peroxidation.
