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Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
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Cell type-selective imaging and profiling of newly synthesized proteomes by using puromycin analogues
Shubo Du1, Danyang Wang1, Jun-Seok Lee2
1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore. chmyaosq@nus.edu.sg.
Chemical Communications (Cambridge, England)
|July 14, 2017
Abstract:
We have developed a versatile antibody-assisted strategy for the imaging and profiling of newly synthesized proteomes in a cell-specific manner. This strategy remained highly selective even in heterogeneous co-cultured cells, thus enabling labeling and enrichment of nascent proteomes from targeted cells without the need for physical separation.
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