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[Study on extraction conditions for minute powder of processed rhizome of Corydalis with vinegar]
Yong-xin Zhang1, Ying Zhang, Qi-wei Zhang
1Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Objective:
To examine the factors in extracting the minute powder of processed rhizome of Corydalis with vinegar for its application.
Method:
The one-way variation analysis was used for experimental design. Tetrahydropalmatine was determined with HPLC method. The samples were separated on a Diamonsil C18 column with mobile phase of acetonitrile-TEA phosphate buffer (pH 7.0) (50:50) and detection wavelength was 280 mm.
Result:
The extraction conditions were established as follows: extraction temperature is 60 degrees C, water-added amount is 20 times the weight of the powder, and extraction time is 30 minutes.
Conclusion:
The extraction rate of the tetrahydropalmatine in the minute power is about 10% higher than that of the processed rhizome with vinegar. But less water amount, less time, and lower temperature were needed for extraction. The application of minute powder can reduce energy consumption and raise economic benefit in industrial production.
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