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Published on: April 13, 2019
Metabolomics and polysaccharide fingerprinting reveal compositional differences between cultivated Fritillaria bulbs
Zhao Geng1, Yijie Ren1,2, Zhen Hu2
1Sichuan Institute for Drug Control (Sichuan Testing Center of Medical Devices, Sichuan Institute of Musk Deer Breeding), Chengdu 611731, China.
Abstract:
Although Fritillariae Cirrhosae Bulbus (FCB) is used in food-related product contexts and local botanical-infusion practices, co-produced flowers remain underutilized and poorly characterized. To support QC-oriented screening and consistency evaluation, we compared bulbs and flowers from two dominant cultivated sources, F. unibracteata var. wabuensis (FW) and F. unibracteata (F), across 36 batches. UHPLC-Q-TOF-MS metabolomics annotated 76 compounds and discriminated between cultivated source and tissue, yielding candidate markers. Targeted UHPLC-QQQ-MS quantified five alkaloids; peiminine differed significantly across four sample groups, whereas sipeimine showed a higher distributional tendency in FW flowers, indicating that flower by-products should be evaluated as compositionally distinct materials rather than simple bulb substitutes. GPC-RI-MALS indicated higher mean PDI in flower crude polysaccharides, and a validated UHPLC-QQQ-MS method quantified 12 monosaccharides, of which eight were higher in flowers. This framework provides integrated chemical evidence for FW/F discrimination and a compositional basis for future QC-oriented and food-related evaluation of flower by-products.
