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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Determination of the coding and non-coding nucleotide sequences of genuine Edmonston-Zagreb master seed and current
Marijana Baricević1, Dubravko Forcić, Tanja Kosutić Gulija
1Institute of Immunology Inc., Molecular Biomedicine Unit, Rockefeller Street 10, 10000 Zagreb, Croatia.
Abstract:
To confirm the genetic stability of the Edmonston-Zagreb vaccine strain, we determined and compared the nucleotide sequences of genuine Edmonston-Zagreb master seed (EZ D22) and current working seed lot (EZ D24 2/99). Sequence analysis and comparison of the two sequences confirmed that these two sequences are the same at the molecular level. The obtained sequences were also compared to reference strains, i.e. Edmonston wild-type (Edmonston Wt) AF266288 and Edmonston-Zagreb (EZ) AF266290 vaccine strain. The sequence of EZ D22 differed from the Edmonston Wt in 32 nucleotides. EZ D22 differed from EZ AF266290 in six nucleotides. Coding substitution at position 441 and two silent substitutions at positions 11999 and 14612 in the L gene are unique to EZ D22. The differences found between EZ from different sources can be a good reason for periodical sequence analysis of the same strain in the hands of different manufactures.
