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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Androgen receptor coregulator ARA267-alpha interacts with death receptor-6 revealed by the yeast two-hybrid

Tiejun Mai1, Xin Wang, Zhiwen Zhang

  • 1Institute of Urology, First Hospital of Peking University, Beijing 100034, China.

Science in China. Series C, Life Sciences
|December 30, 2004
PubMed

Insights

Researchers discovered a new protein, ARA267-alpha, that interacts with death receptor-6 (DR6). This interaction suggests a link between androgen signaling and apoptosis pathways in cells.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Cancer Research

Background:

  • Androgen receptor (AR) coactivators play crucial roles in gene regulation.
  • Death receptor-6 (DR6) is a TNF receptor family member involved in apoptosis.
  • Understanding AR coactivator interactions is key to deciphering cellular signaling networks.

Purpose of the Study:

  • To identify proteins interacting with the conserved domains of ARA267-alpha.
  • To investigate the functional relationship between ARA267-alpha and its interacting partners.
  • To explore potential cross-talk between androgen signaling and apoptosis.

Main Methods:

  • Yeast two-hybrid screening using ARA267-alpha fragment (PHD-SET domains) as bait.
  • In vitro and in vivo interaction assays to confirm binding between ARA267-alpha and DR6.
  • Analysis of DR6's death domain (DR6cp) for mediating apoptosis.

Main Results:

  • Identified death receptor-6 (DR6) as an interacting protein with the ARA267-alpha PHD-SET fragment.
  • Confirmed the interaction between ARA267-alpha-PHD-SET and DR6's cytoplasmic portion (DR6cp).
  • Demonstrated interaction occurs both in vitro and in vivo.

Conclusions:

  • ARA267-alpha interacts with DR6, a key mediator of apoptosis.
  • This interaction suggests a novel cross-talk mechanism between the androgen signaling pathway and the apoptosis signaling pathway.
  • The findings provide new insights into the regulation of cell death by androgen signaling.

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