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Related Experiment Videos

CD98hc (SLC3A2) mediates integrin signaling.

Chloe C Feral1, Naoyuki Nishiya, Csilla A Fenczik

  • 1Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Proceedings of the National Academy of Sciences of the United States of America
|December 31, 2004
PubMed
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CD98hc protein is crucial for integrin signaling, promoting cell migration, survival, and tumor growth. Its interaction with integrins, independent of amino acid transport, drives tumorigenesis.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Integrins are key regulators of cellular functions via signaling pathways.
  • CD98 heterodimers, including CD98hc (SLC3A2), interact with integrins.
  • CD98hc overexpression is linked to cell growth and tumorigenesis.

Purpose of the Study:

  • To elucidate the biological role of CD98hc by analyzing CD98hc-null cells.
  • To determine CD98hc's contribution to integrin-dependent signaling and cellular behaviors.
  • To investigate CD98hc's role in tumorigenesis.

Main Methods:

  • Gene disruption to create CD98hc-null cells.
  • Analysis of cell spreading, migration, and apoptosis in CD98hc-null cells.
  • Assessment of Akt and Rac GTPase activation upon cell adhesion.

Related Experiment Videos

  • Utilizing a CD98hc mutant to differentiate integrin interaction from amino acid transport.

    • Main Results:

    • CD98hc is essential for integrin-dependent cell spreading, migration, and apoptosis resistance.
    • CD98hc mediates adhesion-induced activation of Akt and Rac GTPase.
    • A CD98hc mutant interacting with beta1 integrins, but not light chains, rescued signaling and survival.
    • CD98hc deletion impaired teratocarcinoma formation in mice, which was restorable by CD98hc or the integrin-binding mutant.

    Conclusions:

    • CD98hc functions as an integrin-associated protein mediating critical integrin-dependent signals.
    • These signals, including those regulating cell survival and migration, are vital for tumorigenesis.
    • CD98hc's role in integrin signaling, rather than amino acid transport, is key to its pro-tumorigenic function.