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[Polymorphism analysis of MHC-DRB3 gene in Dolang sheep with PCR-RFLP]
Yun-Fang Liu1, Gen-Qiang Yan, Xin-Feng Wang
1College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. lyf_6@Yahoo.com.cn
Abstract:
MHC is a chromosomal region consisting of a group of closely linked loci which are highly polymorphic, and plays a central role in the immune system. The restrictive polymorphism of MHC-DRB3 exon2 in Dolang sheep was Analyzed by PCR-RFLPs. The results revealed extensive polymorphisms 2, 2 and 6 RFLP types of PCR products were found with enzymes TaqI, PstI and HaeIII respectively. Considering all restrictive pattern, 24 alleles for DRB3 locus were found in Dolang sheep.
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