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[Effect of osmoregulation on the plasma membrane redox activity in chilling injury sensitive soybean seeds]
Yong-Qing Yang1, Xiao-Feng Wang, Shu-Hua Wang
1Department of Biology, Beijing Forestry University, Beijing 100083, China.
Abstract:
Soybean (Glycine max Heihe No.23) seeds sensitive to imbibitional chilling injury were used to systematically investigate the effects of osmoregulation on the plasma membrane redox activity. Positive correlation was found between chilling tolerance and the plasma membrane redox activity. Osmoregulation pretreatment for 12 h significantly improved tolerance of seeds toward imbibitional chilling, the germination index, vigor index and TF content were increased, NADH and NADPH oxidation rates, Fe(CN)(3-)(6) and EDTA-Fe(3+) reduction rates were increased. Chilling tolerance of seeds increased as with duration of osmoregulation, and the rates of NADH oxidation, NADPH oxidation and Fe(CN)(3-)(6) reduction were improved, EDTA-Fe(3+) reduction rate was decreased after osmoregulation for 24 h, but higher than the control seed (seed not with osmoregulation).
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