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Updated: Aug 20, 2026
Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)
Published on: April 28, 2007
[Expression of HSP70 in human umbilical vein endothelial cells induced by Hantavirus]
Lu Yu1, Heng Ma, Shou-Jing Yang
1Department of Pathology ,Fourth Military Medical University, Xi'an 710032, China.
Aim:
To study stress response in human umbilical vein endothelial cells (HUVEC) infected with Hantavirus (HTV).
Methods:
The expression of HSP70 was detected by immunocytochemical staining, in situ nucleic acid hybridization and RT-PCR.
Results:
After being infected with HTV, the expression of HSP70 in HUVEC increased. RT-PCR showed that the level of HSP70 mRNA increased rapidly and kept at a high level in comparison with the normal control (P<0.05).
Conclusion:
The over-expression of HSP70 in HUVECs can be induced by Hantavirus infection, which may play a role in the inhibition of virus reproduction and the protection of cells from virus infection.
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