Related Experiment Video
Updated: Aug 20, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Published on: September 2, 2019
[Identification of QTLs on substituted segments in single segment substitution lines of rice]
Guan-Ming Liu1, Wen-Tao Li, Rui-Zhen Zeng
1Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
Abstract:
The quantitative trait loci (QTLs) for 21 traits of agronomy importance on ten substituted chromosomal segments were identified using single segment substitution lines (SSSLs) in rice, which were developed by the use of Taichung 65 as a recipient and zhai-ye-qing and dee-geo-woo-gen as donors respectively. Total length of the substituted segments in the SSSLs was 230.00 cM, which was 12.62% of rice genome. T-test was used to detect QTLs in the condition that the difference in phenotypes between a SSSL and the recipient parent (Taichung 65) was significant at the 0.1% level (P< or = 0.001). A total of 57 QTLs for 17 traits had been detected on the ten substituted segments, which were on chromosomes 1, 3, 5, 7, 8, 10 and 12 respectively. The additive effect percentages of the QTLs ranged from 1.10% to 89.73%. Among of 57 QTLs, fifteen QTLs had the additive effect percentage over 10%, thirty QTLs varied from 3% to 10% and other twelve QTLs were below 3%.

