Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exosite-driven substrate specificity and function in coagulation.

S Krishnaswamy1

  • 1Joseph Stokes Research Institute, Children's Hospital of Philadelphia & Department of Pediatrics, University of Pennsylvania, Philadelphia, PA 19104, USA. skrishna@mail.med.upenn.edu

Journal of Thrombosis and Haemostasis : JTH
|January 7, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Information theoretic perspective on genome clustering.

Saudi journal of biological sciences·2021
Same author

The barriers to linkage and retention in care for women living with HIV in an high income setting where they comprise a minority group.

AIDS care·2019
Same author

Optimised dosing of vancomycin in critically ill Indigenous Australian patients with severe sepsis.

Anaesthesia and intensive care·2018
Same author

The rise of methicillin resistant Staphylococcus aureus: now the dominant cause of skin and soft tissue infection in Central Australia.

Epidemiology and infection·2017
Same author

Understanding the virulence of the entero-aggregative E. coli O104:H4.

International journal of bioinformatics research and applications·2015
Same author

Variations in physicochemical properties of a traditional mercury-based nanopowder formulation: need for standard manufacturing practices.

Indian journal of pharmaceutical sciences·2015
Same journal

The Natural Mutation Arg221aTrp in Human α-Thrombin Abrogates Physiological Na<sup>+</sup> Binding and Preferentially Hinders the Protease Anticoagulant Functions.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

A historical review of the biological, semantic and clinical aspects of aspirin resistance.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Association between Thrombus Neutrophil Extracellular Traps Content and Ischemic Stroke Recurrence.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Peptide-Mediated Inhibition of Surface-Initiated Thrombogenesis.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Growth differentiation factor-15 and bleeding risk in patients with venous thromboembolism.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Physiological Anticoagulant Deficiencies: Pathogenesis, Diagnosis, and Clinical Implications.

Journal of thrombosis and haemostasis : JTH·2026
See all related articles

Prothrombinase uses exosites to achieve high substrate specificity, challenging previous models. This exosite-driven mechanism is crucial for blood coagulation and other enzymes acting on protein substrates.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Serine proteinase specificity is critical for hemostasis.
  • Digestion enzyme models have influenced coagulation enzyme research.
  • Developing coagulation inhibitors relies on understanding enzyme specificity.

Purpose of the Study:

  • To investigate the basis of narrow substrate specificity in coagulation enzymes.
  • To challenge prevailing models of enzyme specificity.
  • To explore the role of exosites in prothrombinase function.

Main Methods:

  • Enzymology studies
  • Physical biochemistry of prothrombinase
  • Analysis of substrate recognition mechanisms

Related Experiment Videos

Main Results:

  • Prothrombinase utilizes exosites for substrate recognition and directed action.
  • Exosite-driven mechanisms are key to achieving narrow protein substrate specificity.
  • This strategy is applicable to other coagulation reactions and enzyme families.

Conclusions:

  • Exosite-driven enzymic function is a fundamental strategy for high macromolecular substrate specificity.
  • This challenges traditional views based on digestive enzymes.
  • Understanding exosites is vital for both basic research and therapeutic development.