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Related Experiment Videos

Factor H binds to washed human platelets.

F Vaziri-Sani1, J Hellwage, P F Zipfel

  • 1Department of Pediatrics, Lund University, Lund, Sweden.

Journal of Thrombosis and Haemostasis : JTH
|January 7, 2005
PubMed
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Factor H binds to platelets through its C-terminus, either directly via the GPIIb/IIIa receptor or indirectly via thrombospondin-1. This novel platelet interaction occurs independently of complement, offering new insights into Factor H function.

Area of Science:

  • Immunology
  • Hematology
  • Complement System Biology

Background:

  • Factor H regulates the alternative complement pathway and possesses heparin-binding sites.
  • Mutations in Factor H's C-terminus are linked to atypical hemolytic uremic syndrome, a condition involving platelet consumption.
  • Factor H has been observed to copurify with thrombospondin-1 from platelets.

Purpose of the Study:

  • To investigate the interaction between Factor H and platelets.
  • To determine the specific regions of Factor H involved in platelet binding.
  • To elucidate the mechanisms of Factor H-platelet interaction.

Main Methods:

  • Flow cytometry was used to analyze the binding of Factor H and its constructs to washed platelets.
  • Surface plasmon resonance measured the binding of Factor H to thrombospondin-1.

Related Experiment Videos

  • Recombinant C- and N-terminus constructs, along with a C-terminus mutant of Factor H, were employed.

    • Main Results:

    • Factor H demonstrated dose-dependent binding to platelets.
    • The C-terminus of Factor H was identified as the primary binding site.
    • Platelet GPIIb/IIIa receptor and thrombospondin-1 were implicated in mediating Factor H binding.
    • A C-terminus mutated Factor H exhibited significantly reduced platelet binding.

    Conclusions:

    • Factor H exhibits novel binding to platelets, independent of complement activation.
    • This interaction can occur directly via the GPIIb/IIIa receptor or indirectly through thrombospondin-1.
    • The C-terminal region of Factor H is crucial for its interaction with platelets.