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Related Experiment Videos

A quantitative high-throughput endothelial cell migration assay.

Vladimir Mastyugin1, Elizabeth McWhinnie, Mark Labow

  • 1Novartis Institutes for Biomedical Research, 250 Massachusetts Avenue, Cambridge, MA, USA.

Journal of Biomolecular Screening
|January 7, 2005
PubMed
Summary

A new cell migration assay using FluoroBlok Boyden chambers and Cellomics HCS ArrayScan offers a 10-fold improvement in sensitivity. This method accurately measures endothelial cell migration and screens compounds inhibiting growth factor effects.

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Pharmacology

Background:

  • Cell migration is crucial for biological processes like development and wound healing.
  • Existing cell migration assays often lack sensitivity and rely on less precise fluorescence measurements.
  • Developing more sensitive assays is vital for understanding cell behavior and drug discovery.

Purpose of the Study:

  • To develop a novel, highly sensitive cell migration assay.
  • To improve the signal-to-background ratio compared to conventional methods.
  • To enable robust assessment of growth factor effects and compound screening.

Main Methods:

  • Combined BD Biosciences FluoroBlok membrane-based Boyden chambers with Cellomics HCS ArrayScan.
  • Developed a novel assay based on counting nuclei of migrated cells.

Related Experiment Videos

  • Compared the new method to conventional total well fluorescence measurements.

    • Main Results:

    • The new assay demonstrated approximately 10-fold increased signal/background ratio.
    • Successfully measured endothelial cell migration in response to growth factors.
    • Validated the assay for screening chemical compounds for inhibitory effects.

    Conclusions:

    • The developed assay provides a significantly more sensitive and accurate method for measuring cell migration.
    • This technique is suitable for studying growth factor-induced endothelial cell migration.
    • The assay is effective for high-throughput screening of compounds targeting cell migration pathways.