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Related Experiment Videos

DNA aggregating protein from carp (Cyprinus capio) eggs.

Masahiro Hosono1, Shigeki Sugawara, Yukiko Ogawa

  • 1Cancer Research Institute, Tohoku Pharmaceutical University, Sendai, Japan.

Biological & Pharmaceutical Bulletin
|January 7, 2005
PubMed
Summary

Researchers discovered a novel DNA-aggregating protein (DAP) in carp eggs that binds to DNA and causes hemagglutination. This non-histone protein, distinct from nucleohistones, shows partial homology to HU-alpha DNA-binding protein.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Ichthyology

Background:

  • Carp egg extracts exhibit hemagglutinating activity.
  • This activity is inhibited by sulfated glycans and DNA, suggesting molecular interactions.

Purpose of the Study:

  • To isolate and characterize the molecules responsible for hemagglutinating and DNA-binding activities in carp egg extract.
  • To determine if hemagglutination and DNA aggregation activities are mediated by the same or different molecules.

Main Methods:

  • Hydroxyapatite and heparin-Sepharose column chromatography for protein purification.
  • Dot blot and Southwestern blot analyses for DNA binding.
  • N-terminal amino acid sequencing.

Main Results:

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  • A novel 18 kDa DNA-aggregating protein (DAP) was identified.
  • DAP exhibits hemagglutinating activity and binds to DNA, including short oligonucleotides.
  • Hemagglutination and DNA aggregation activities could be separated, with HS3 showing highest DNA aggregation and HS2/HS4 showing higher hemagglutination.
  • DAP's N-terminal sequence shows partial homology to HU-alpha DNA-binding protein, but not to nucleohistones.

Conclusions:

  • Carp eggs contain a novel, non-histone DNA-aggregating protein (DAP).
  • This DAP possesses both hemagglutinating and DNA-binding properties.
  • The findings suggest a unique DNA-binding protein in fish eggs with potential roles in early development or defense.