Simultaneous determination of dopamine and ascorbic acid at a poly(toluidine blue) modified electrode

Area of Science:

• Electrochemistry
• Materials Science
• Analytical Chemistry

Background:

• Dopamine (DA) and ascorbic acid (AA) are crucial biomolecules.
• Accurate electrochemical detection and discrimination of DA and AA are challenging.
• Novel electrode materials are needed for sensitive and selective analysis.

Purpose of the Study:

• To develop a novel polymer-modified electrode for electrochemical sensing.
• To investigate the electrode's catalytic activity towards dopamine and ascorbic acid.
• To establish a method for the simultaneous determination and discrimination of DA and AA.

Main Methods:

• Fabrication of a poly(toluidine blue) modified electrode.
• Electrochemical oxidation studies of dopamine and ascorbic acid.
• Utilizing semi-derivative voltammetry for signal analysis.
• Testing the electrode's performance in brain tissue samples.

Main Results:

• The modified electrode exhibited remarkable catalytic activity for DA and AA oxidation.
• Semi-derivative voltammograms clearly distinguished between DA and AA electrochemical signals.
• Achieved linear detection ranges: DA (0.4 µmol L⁻¹–1.5 mmol L⁻¹), AA (0.2 µmol L⁻¹–2.4 mmol L⁻¹).
• Successful tentative determination of DA and AA in brain tissue.

Conclusions:

• The poly(toluidine blue) modified electrode is a promising material for electrochemical sensing.
• The developed method allows for sensitive and selective simultaneous detection of DA and AA.
• This approach holds potential for analyzing neurotransmitters and related compounds in biological samples.