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Direct comparison between two 1-84PTH assays in dialysis patients
Junichiro J Kazama1, Suguru Yamamoto, Shigemi Kameda
1Division of Clinical Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan. jjkaz@med.niigata-u.ac.jp <jjkaz@med.niigata-u.ac.jp>
Nephron. Clinical Practice
|January 8, 2005
Summary
Two types of 1-84 intact parathyroid hormone (PTH) assays show high correlation in dialysis patients. While generally comparable for clinical practice, the 1-84 PTH/(intact PTH - 1-84 PTH) ratio requires careful application due to amplified assay errors.
Area of Science:
- Clinical Chemistry
- Endocrinology
- Renal Medicine
Background:
- Two distinct 1-84 intact parathyroid hormone (PTH) assays are currently utilized in clinical settings.
- Limited research has directly compared the performance of these assays using the same plasma samples.
Purpose of the Study:
- To evaluate and compare the results of two commercially available 1-84 intact parathyroid hormone (PTH) assays.
- To assess the agreement between these assays in classifying dialysis patients according to clinical guidelines.
Main Methods:
- Plasma samples from 235 dialysis patients were analyzed.
- Simultaneous analysis was performed using two 1-84 intact PTH assays (IRMA and CLIA) and two intact PTH assays (IRMA and CLIA).
Main Results:
- The 1-84 intact PTH-IRMA and 1-84 intact PTH-CLIA assays demonstrated a high degree of correlation (r² = 0.971).
- Assay agreement for classifying patients into three K/DOQI categories ranged from 90.2% to 92.3%.
- Agreement decreased significantly (41.3-83.4%) when classifying patients into two categories using the 1-84 PTH/(intact PTH - 1-84 PTH) ratio.
Conclusions:
- The two 1-84 intact parathyroid hormone (PTH) assays provide comparable results for routine clinical practice.
- The calculated ratio of 1-84 PTH/(intact PTH - 1-84 PTH) should be used cautiously as it can magnify assay inaccuracies.