[Polymorphism analysis of porcine myogenin gene by PCR-RFLP]

The polymorphisms of porcine myogenin gene in 561 pigs including Duroc, Landrace, Large Yorkshire, Nanchang white pig, Erhualian, Meishan, Yushan black pig, Leping spotted pig, Jinhua black head-hind pig and Shanggao black head-hind pig were detected by PCR-RFLPs with three different pairs of primers,and the PCR products were digested by MspI. The results showed that most of the Duroc, Landrace, Large Yorkshire, Nanchang white pigs presented as AA genotype, while more animals of the six Chinese local pig breeds except for Leping spotted pig presented as BB genotype at PCR1 MspI-RFLP site. The six Chinese local breeds presented as MM genotype except that one Yushan black pig presented as MN genotype, while more swines of the exotic breeds including Duroc, Landrace, Large Yorkshire presented as NN genotype, and Nanchang white pig appeared to be closer to the exotic breeds at PCR2 MspI-RFLP site. PCR product was obtained in all the swine by PCR3, but the MspI restriction site was not found in the tested pig breeds including Meishan and blood closely related Erhualian pig.