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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Updated: Jun 25, 2026

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

[A novel method of extracting total RNA from rice embryo samples].

Wen-Biao Shen¹, Ren Wang, Yi-Hua Wang

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement, Rice Research Institute, Nanjing Agricultural University, Nanjing 210095, China. wanjm@njau.edu.cn

Yi Chuan = Hereditas

|January 11, 2005

Summary

This summary is machine-generated.

A new method efficiently extracts high-quality RNA from rice embryos, even with high polysaccharide and lipid content. This room-temperature technique simplifies RNA isolation for plant research.

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Last Updated: Jun 25, 2026

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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

Area of Science:

Molecular Biology
Plant Science
Biochemistry

Context:

Rice (Oryza sativa L.) embryos are rich in polysaccharides and lipids, complicating total RNA extraction.
Traditional RNA extraction methods often require liquid nitrogen and may be inefficient for recalcitrant plant tissues.
Accurate RNA quantification and integrity assessment are crucial for downstream molecular analyses.

Purpose:

To develop a novel, simple, and efficient method for extracting high-quality total RNA from rice embryo samples.
To overcome the challenges posed by polysaccharides and lipids in rice embryo RNA isolation.
To provide a reliable RNA extraction protocol suitable for plant materials abundant in polysaccharides and lipids.

Summary:

A CTAB-LiCl-based method was developed for total RNA extraction from rice embryos at room temperature.
This protocol effectively removes polysaccharide and lipid contaminants, yielding high-purity RNA.
Analysis via spectrophotometry and gel electrophoresis confirmed RNA integrity and purity, suitable for RT-PCR and RNA gel blotting.

Impact:

Enables efficient RNA extraction from challenging plant samples, improving accessibility for researchers.
Provides a cost-effective and simplified alternative to conventional RNA isolation techniques.
Facilitates downstream gene expression studies and molecular diagnostics in rice and other polysaccharide/lipid-rich plants.