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Updated: Aug 20, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
[Cloning and sequencing of Sry gene of muntiacus]
Xiao-Xuan Lu1, Yue Zhang, Xiang-Nian Shan
1Genetic Research Center, Medical College of Southeast University, Nanjing 210009, China. luxiaoxuan@hotmail.com
Abstract:
Using the primers from SRY gene--HMG Box for PCR amplification in genomic DNA of Muntiacus reevesi cell strains, a 220bp fragment was obtained in the male but not in the female. The 220bp fragment was cloned into the pGEM-T vector using T/A clone method. The identified positive clone was sequenced. The result shows that 82.6% nucleotides(152bp/184bp) are homologous between Muntiacus Sry and human SRY gene. It suggests that SRY is highly conserved during evolution.
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