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[Activation tagging and the application in plant genetic engineering]
Ji-Gang Zheng1, Cheng-Mei Li, Ying-Hua Xiao
1Department of Biology, Capital Normal University, Beijing 100037, China.
Yi Chuan = Hereditas
|January 11, 2005
Abstract:
Activation tagging is a new method for isolation and functional identification. It can generate dominant gain-of-function mutants by overexpression of a particular endogenous gene. Due to this special characteristics of activation tagging,this method has been a powerful tool for new gene discovery and gene functional analysis. This paper reviewed the principle and study conditions of activation tagging,as well as its use in plant genetic engineering.