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Updated: Aug 20, 2026

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina
Published on: May 6, 2015
Role of Ca2+ store in AMPA-triggered Ca2+ dynamics in retinal horizontal cells
Shi-Yong Huang1, Yu Liu, Pei-Ji Liang
1Key Laboratory of Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, USA.
Abstract:
Fura-2 fluorescent calcium imaging was applied to measure [Ca(2+)](i) in freshly dissociated horizontal cells of carp retina, and a model containing endoplasmic reticulum (ER) membrane processes and plasma membrane processes was constructed for quantitative analyses of the AMPA-triggered calcium dynamics. A transient increase followed by a sustained steady level of [Ca(2+)](i) was observed when 100 microM AMPA was applied, while the initial transient increase of [Ca(2+)](i) was suppressed by exogenously applied ryanodine. The model analyses results suggest that the AMPA-triggered calcium dynamics involves a number of cytoplasmic and endoplasmic processes that interact with each other. It also suggests that calcium store is an important part contributing to the transient calcium signal.
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