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Radioimmunoimaging. Advances and prospects.
C Van de Wiele1, H Revets, N Mertens
1Department of Nuclear Medicine, University Hospital, Ghent, Belgium. christophe.vandewiele@ugent.be
Summary
Biotechnology advances enable the creation of fully human antibodies, overcoming immune responses. New techniques also allow for tailored antibody affinity, avidity, and smaller, stable protein binders for improved applications.
Area of Science:
- Biotechnology
- Immunology
- Protein Engineering
Background:
- Rodent antibodies for radioimmunoimaging can cause host antiglobulin responses.
- Previous solutions included chimeric and CDR-grafted antibodies.
- Need for improved antibody-based therapeutics and diagnostics.
Purpose of the Study:
- To review advances in antibody engineering to overcome host immune responses.
- To discuss methods for tailoring antibody affinity and avidity.
- To highlight novel antibody derivatives and pretargeting strategies.
Main Methods:
- Derivation of completely human antibodies using phage display, ribosome display, and transgenic mice.
- Affinity tailoring via CDR-walking and chain shuffling.
- Avidity engineering through manifold approaches.
- Development of single domain binders and pretargeting technologies.
Main Results:
- Successful generation of human antibodies, reducing immunogenicity.
- Ability to precisely engineer antibody affinity and avidity.
- Emergence of smaller, stable protein binders as alternatives.
- Advancements in pretargeting strategies for enhanced applications.
Conclusions:
- Biotechnology has revolutionized antibody development, minimizing immune rejection.
- Engineered antibodies and novel binders offer improved therapeutic and diagnostic potential.
- Ongoing innovations in antibody technology promise further advancements in the field.