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High-performance liquid chromatographic method using ultraviolet detection for measuring metrifonate and dichlorvos
L K Unni1, M E Hannant, R E Becker
1Department of Psychiatry, Southern Illinois University School of Medicine, Springfield 62702.
Journal of Chromatography
|January 3, 1992
Abstract:
We report high-performance liquid chromatographic methods using ultraviolet detection, developed for the first time in our laboratory with sensitivity to detect clinically significant concentrations of metrifonate (MTF), an experimental drug for Alzheimer disease, and its active anticholinesterase metabolite, dichlorvos (DDVP). The determination limit of the method for MTF and DDVP was 1 microgram/ml and 40 ng/ml, respectively. Stability of MTF and DDVP at various temperatures in water, buffered solutions and in human plasma were also studied.