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Microtiter Dish Biofilm Formation Assay
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Inhibition of biofilm formation by monoclonal antibodies against Staphylococcus epidermidis RP62A

Daqian Sun1, M A Accavitti, J D Bryers

  • 1Department of Chemical Engineering, University of Connecticut, Storrs, Connecticut, USA.

Clinical and Diagnostic Laboratory Immunology
|January 12, 2005
PubMed

Insights

Monoclonal antibodies targeting Staphylococcus epidermidis accumulation-associated protein (AAP) significantly reduced bacterial biofilm formation on surfaces. Combinations of antibodies were most effective, showing up to 87% reduction in S. epidermidis colonization.

Area of Science:

  • Microbiology
  • Immunology
  • Biotechnology

Background:

  • Staphylococcus epidermidis forms biofilms on biomedical devices, mediated by the cell wall-bound accumulation-associated protein (AAP).
  • Targeting AAP with antibodies presents a potential strategy to prevent S. epidermidis colonization and biofilm formation.

Purpose of the Study:

  • To isolate AAP and generate monoclonal antibodies (MAbs) against it.
  • To evaluate the efficacy of these MAbs in inhibiting S. epidermidis biofilm formation.

Main Methods:

  • Isolation of AAP and generation of MAbs.
  • Testing polyclonal and monoclonal antibodies against S. epidermidis biofilm formation.
  • Characterization of a protein from the S. epidermidis M7 mutant using mass spectrometry.

Main Results:

  • Polyclonal antibodies reduced biofilm formation by 54%.
  • Individual MAbs reduced biofilm formation by up to 66%.
  • MAb mixtures (12C6/12A1 and 3C1/12A1) achieved 79% and 87% reduction, respectively.
  • The S. epidermidis M7 mutant expressed a 200-kDa protein with 53% homology to AAP.

Conclusions:

  • Monoclonal antibodies, particularly in combination, are effective in inhibiting S. epidermidis biofilm formation.
  • The M7 mutant's 200-kDa protein warrants further investigation for its role in biofilm development.

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