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Updated: Aug 20, 2026

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
Published on: April 22, 2022
5-hydroxyuracil can form stable base pairs with all four bases in a DNA duplex
Varatharasa Thiviyanathan1, Anoma Somasunderam, David E Volk
1Department of Human Biological Chemistry & Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA.
Abstract:
NMR studies, UV-monitored melting experiments, and ab initio calculations show that 5-hydroxyuracil, produced by the oxidative de-amination of cytosines by reactive oxygen species, can form stable base-pairs with dA, dG, dC and dT residues in a DNA duplex, providing a basis for the in-vivo incorporation of 5-hydroxyuracil during DNA replication.
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