Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative NAT for pathogen inactivation verification.

K McKenney1, L Gillmeister, K Marlowe

  • 1Clearant Inc., Gaithersburg, MD 20879, USA. kmckenney@clearant.com

Developments in Biologicals
|January 14, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Innovative ways of treating comorbid diabetes type II and depression: piloting the 'MADE-IT' program.

Acta neuropsychiatrica·2016
Same author

Expression of metalloproteases by cardiac myocytes and fibroblasts in vitro.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
Same author

Cardiac integrins the ties that bind.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
Same author

Evolution of Synthyris sect. Dissecta (Plantaginaceae) on sky islands in the Northern Rocky Mountains.

American journal of botany·2011
Same author

Differential expression of miRNAs in the visceral adipose tissue of patients with non-alcoholic fatty liver disease.

Alimentary pharmacology & therapeutics·2010
Same author

Viability and function in lymphocytes cultured from the horse, chicken, and mouse: effects of different leukocyte enrichment techniques.

Journal of immunoassay & immunochemistry·2008
Same journal

Review of Ebola virus infections in domestic animals.

Developments in biologicals·2013
Same journal

Ebola: facing a new transboundary animal disease?

Developments in biologicals·2013
Same journal

Opportunities in diagnostic and vaccine approaches to mitigate potential heartwater spreading and impact on the American mainland.

Developments in biologicals·2013
Same journal

Q fever diagnosis and control in domestic ruminants.

Developments in biologicals·2013
Same journal

Schmallenberg virus.

Developments in biologicals·2013
Same journal

Classical swine fever.

Developments in biologicals·2013
See all related articles

A new Quantitative Nucleic Acid Test (Q-NAT) accurately measures pathogen inactivation from methods like gamma irradiation. This advanced technology offers a more cost-effective and efficient alternative to traditional cell culture detection methods.

Area of Science:

  • Biotechnology
  • Microbiology
  • Molecular Biology

Background:

  • Traditional methods for detecting pathogen inactivation, such as cell culture, are often time-consuming and labor-intensive.
  • There is a need for more efficient and broadly applicable methods to verify pathogen inactivation, especially for nucleic acid-targeting treatments like gamma irradiation.

Purpose of the Study:

  • To introduce and validate a novel Quantitative Nucleic Acid Test (Q-NAT) technology.
  • To demonstrate the capability of Q-NAT in quantifying and verifying pathogen nucleic acid breakdown.
  • To compare the advantages of Q-NAT against conventional detection methods.

Main Methods:

  • Development of a novel Quantitative Nucleic Acid Test (Q-NAT).
  • Application of Q-NAT to assess pathogen inactivation.

Related Experiment Videos

  • Specific focus on methods that break pathogen nucleic acids, such as gamma irradiation.
  • Comparison with traditional cell culture methods for pathogen detection.

    • Main Results:

    • The developed Q-NAT technology effectively demonstrates and quantifies pathogen inactivation.
    • Q-NAT provides verification of nucleic acid breakdown by inactivation methods.
    • Significant advantages in cost and efficiency were observed for Q-NAT compared to cell culture.

    Conclusions:

    • Quantitative Nucleic Acid Test (Q-NAT) is a novel and effective technology for pathogen inactivation assessment.
    • Q-NAT offers superior cost-effectiveness, efficiency, and broad applicability over traditional methods.
    • This technology advances the verification of pathogen inactivation, particularly for nucleic acid-disrupting treatments.