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Related Experiment Videos

Application of solid-phase microextraction to in vitro skin permeation experiments: example using diethyl phthalate.

H Fred Frasch1, Ana M Barbero

  • 1Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA. hbf9@cdc.gov

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|January 15, 2005
PubMed
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Automated solid-phase microextraction (SPME) effectively prepares samples for analyzing diethyl phthalate (DEP) skin permeation. This method offers a sensitive and precise approach for in vitro skin absorption studies.

Area of Science:

  • Pharmacokinetics and Drug Delivery
  • Analytical Chemistry
  • Dermal Toxicology

Background:

  • In vitro skin permeation studies require precise quantification of low-level analytes in aqueous samples.
  • Traditional sample preparation methods can be time-consuming and less efficient for complex matrices like skin extracts.
  • Diethyl phthalate (DEP) is a common chemical exposure, and understanding its dermal absorption is crucial.

Purpose of the Study:

  • To describe the application of automated solid-phase microextraction (SPME) for sample preparation in in vitro skin permeation studies.
  • To evaluate the efficacy of SPME for the quantitative analysis of DEP in skin penetration experiments.
  • To determine key DEP skin permeation parameters using optimized SPME methodology.

Main Methods:

Related Experiment Videos

  • Automated solid-phase microextraction (SPME) was optimized for sample preparation.
  • In vitro diffusion cell experiments were conducted using dermatomed male hairless guinea pig skin.
  • Gas chromatography was used for quantitative analysis of DEP.
  • Skin permeation parameters including permeability coefficient (k(p)), time lag (tau), and skin-buffer partition coefficient (K(SB)) were determined.

    • Main Results:

    • Optimized automated SPME demonstrated adequate sensitivity and good precision (RSD=3%).
    • Key DEP skin permeation parameters were quantified: k(p) = 0.021±0.012 cm/h, tau = 0.67±0.18 h, and K(SB) = 4.74±0.68.
    • The results indicate that skin may represent a significant absorption pathway for DEP.

    Conclusions:

    • Automated SPME is a suitable and advantageous sample preparation technique for in vitro skin permeation studies.
    • The study provides quantitative data on DEP skin permeation, highlighting the potential for dermal uptake.
    • This methodology can be applied to assess the dermal absorption of other chemicals in various in vitro models.