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Proteomics analysis of human cerebrospinal fluid
Xianglin Yuan1, Dominic M Desiderio
1Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Health Science Center, 847 Monroe Avenue, Room 117, Memphis, TN 38163, USA.
Summary
Proteomics analysis of cerebrospinal fluid (CSF) reveals changes in protein composition, reflecting central nervous system (CNS) pathology. This review covers methods and identified proteins for understanding CNS diseases.
Area of Science:
- Neuroscience
- Biochemistry
- Analytical Chemistry
Background:
- Cerebrospinal fluid (CSF) composition changes indicate central nervous system (CNS) pathological processes.
- Proteomics provides a systems-level view of CSF proteins for disease analysis.
Purpose of the Study:
- To review analytical methods for cerebrospinal fluid (CSF) proteomics.
- To discuss differentially expressed CSF proteins identified through proteomics.
Main Methods:
- Sample preparation techniques for CSF.
- Two-dimensional liquid and gel electrophoresis.
- Mass spectrometry and bioinformatics approaches.
- Non-gel-based proteomics methods.
Main Results:
- Overview of various proteomics methodologies applied to CSF.
- Identification of specific CSF proteins with altered expression levels in pathological conditions.
Conclusions:
- Proteomics is a powerful tool for analyzing CSF protein profiles.
- Understanding CSF proteomic changes aids in diagnosing and understanding CNS diseases.