[In vitro long-term culture of human bone under physiological load conditions]

There is evidence that mechanical loading is an important, if not the most important factor influencing bone mass and architecture. Investigations under in vivo conditions and cell culture methods, performed during the last years, helped to elucidate these mechanisms. However, the mechanisms by which load bearing acts on bone tissue are until now not completely understood. It is well accepted that weight-bearing exercise increases bone mass and on the other hand lower physical activity engenders bone loss. But neither a physiological threshold for bone loss or bone growth nor the character of the mechanical stimulus concerning amount, frequency and duration of the applied load are known. Even more speculative is the idea how this signal is transformed into the biological response of growing bone. Three-dimensional bone-culture-systems with simultaneous applied mechanical load enables to improve the knowledge of regulation of bone metabolism. We show the results of a long-term in vitro experiment with human cancellous bone under physiological load conditions.