Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Near-field scanning optical microscopy for bioanalysis at nanometer resolution.

Musundi B Wabuyele1, Mustafa Culha, Guy D Griffin

  • 1Advanced Biomedical Science and Technology Group, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 20, 2005
PubMed
Summary

Near-field scanning optical microscopy (NSOM) enables nondestructive imaging of biomolecules at the nanoscale. This technique visualizes proteins on surfaces, offering insights into their orientation and interactions for biomedical applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Noninvasive whole-brain imaging of glymphatic dynamics.

Science advances·2026
Same author

Machine-learning-based tumor segmentation and classification using dynamic optical contrast imaging for thyroid cancer.

Biophotonics discovery·2026
Same author

Magneto-Plasmonics-Enhanced Colorimetric Lateral Flow Immunoassay Using Magnetic-Gold Nanostars.

ACS applied materials & interfaces·2026
Same author

Direct, Pretreatment-Free Surface-Enhanced Raman Scattering (SERS) Detection of Uric Acid in Urine Facilitated by Efficient Hydrogen Bonding-Driven Capture.

Analytical chemistry·2026
Same author

Rapid Solution-Based SERS Detection of Pesticides Using Graphene Oxide-Coated Silver-Gold Nanostars.

ACS applied nano materials·2026
Same author

Tunable and scalable production of nanostar particle platforms for diverse applications using an AI-integrated automated synthesis system.

Journal of materials science·2026

Area of Science:

  • Biophysics
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Nondestructive imaging of biomolecules at the nanoscale is crucial for understanding their behavior.
  • Existing scanning probe microscopy techniques can disturb molecular orientation and position.
  • Near-field scanning optical microscopy (NSOM) offers high resolution (<100 nm) and a nondestructive approach.

Purpose of the Study:

  • To demonstrate the utility of NSOM for visualizing biomolecules on surfaces at the single-molecule level.
  • To obtain fundamental information on protein orientation and locality without altering their native state.
  • To highlight potential applications in biomedical and biochip fields.

Main Methods:

  • Utilizing Near-field Scanning Optical Microscopy (NSOM) for high-resolution imaging.

Related Experiment Videos

  • Adsorbing or depositing biomolecules onto surfaces for analysis.
  • Applying NSOM to visualize specific proteins like green fluorescent proteins and multidrug resistance proteins.

    • Main Results:

    • Achieved imaging of biomolecules at nanometer domains with <100-nm resolution.
    • Successfully visualized protein orientation and locality on surfaces without disturbance.
    • Demonstrated the feasibility of NSOM for complex biological samples, including those from tumor cells.

    Conclusions:

    • NSOM is a powerful tool for nondestructive, high-resolution imaging of biomolecules.
    • This technique provides critical insights into molecular interactions and behavior on surfaces.
    • NSOM holds significant promise for advancing biomedical research and biochip development.