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Updated: Aug 20, 2026

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
Spectrofluorimetric determination of phenyl-beta-naphthylamine used as rubber antioxidant
Zenovia Moldovan1, Cristina Stoica, Mihaela Hillebrand
1Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd, Cod 030018, Sect 3, Bucharest, Romania. z_moldovan@yahoo.com
Abstract:
Phenyl-beta-naphthylamine (PBN) used as rubber antioxidant was found to have native fluorescence. A spectrofluorimetric method for determination of PBN in multicomponent mixtures of polymer additives is described. The apparent excitation and fluorescence wavelengths used are 348 and 413.5 nm, respectively. Maximum fluorescence intensity is obtained by irradiating PBN dissolved in ethanol, at room temperature. The fluorescence varies linearly with the concentration of PBN in the range of 0.04-4 microg mL(-1). The accuracy and precision of the method are reported.

