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[Determination of alpha-cyperone in Rhizoma Cyperi oil by HPLC]
Congqing Liang1, Yuansheng Luo, Zhaojian Zhong
1Guangdong College of Pharmacy, Guangzhou.
Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials
|January 22, 2005
Abstract
Objective:
To establish a method deteminating alpha-cyperone in Rhizome Cyperi oil by HPLC.
Methods:
The determination was carried out by Merck Lichrocart C18 column, with mobile phase consisted of methanol-water (68: 32), and detective wavelength 241 nm.
Results:
The calibration curves was linear in the range of 0. 081 - 0. 81 microg/ml (r = 0.9998). The average recovery of loading sample was 99.1%, RSD =2.2%.
Conclusion:
The method is convenient for a good resolution.
