Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SARS molecular detection external quality assurance.

Christian Drosten1, Hans Wilhelm Doerr, Wilina Lim

  • 1Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

Emerging Infectious Diseases
|January 25, 2005
PubMed
Summary

External quality assurance for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) testing showed high accuracy. Commercial test kits significantly improved the detection of SARS-CoV RNA in samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biphasic MERS-CoV Incidence in Nomadic Dromedaries with Putative Transmission to Humans, Kenya, 2022-2023.

Emerging infectious diseases·2024
Same author

Distinct phenotype of SARS-CoV-2 Omicron BA.1 in human primary cells but no increased host range in cell lines of putative mammalian reservoir species.

Virus research·2024
Same author

Clinical epidemiology, determinants, and outcomes of viral encephalitis in Ghana; a cross-sectional study.

PloS one·2024
Same author

Immunogenetic-pathogen networks shrink in Tome's spiny rat, a generalist rodent inhabiting disturbed landscapes.

Communications biology·2024
Same author

Humoral immune escape by current SARS-CoV-2 variants BA.2.86 and JN.1, December 2023.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2024
Same author

Deep RNA sequencing of muscle tissue reveals absence of viral signatures in dermatomyositis.

Free neuropathology·2024

Area of Science:

  • Virology
  • Infectious Diseases
  • Diagnostic Assays

Background:

  • Accurate detection of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is critical for public health surveillance.
  • External quality assurance (EQA) programs are essential for validating diagnostic test performance.

Purpose of the Study:

  • To evaluate the performance of diagnostic methods for SARS-CoV detection.
  • To assess the impact of commercial test kits on detection accuracy.

Main Methods:

  • An external quality assurance study was conducted using inactivated SARS-CoV samples.
  • Fifty-eight participants from reference institutions analyzed samples with varying viral loads.
  • Performance was evaluated based on correct detection of SARS-CoV RNA and negative samples.

Related Experiment Videos

Main Results:

  • 51 out of 58 participants accurately detected SARS-CoV in samples with >= 9,400 RNA copies/mL.
  • No participants incorrectly detected SARS-CoV in negative samples.
  • The use of commercial test kits led to a significant improvement in detection outcomes.

Conclusions:

  • Reference laboratories demonstrate high accuracy in detecting SARS-CoV.
  • Commercial test kits enhance the reliability of SARS-CoV diagnostic testing.
  • Continued EQA is vital for maintaining high standards in infectious disease diagnostics.